FIG. 2.

Recruitment of αCP but not hnRNP K into the Lox 3′UTR complex. (A) Identification of the Lox complex by EMSA using a ³²P-labeled Lox 3′UTR probe. The position of the Lox complex (a doublet band) is shown in the first lane, and its sensitivity to poly(C) competition is demonstrated in the following lane. (B) Recruitment of αCP into the Lox complex. Increasing concentrations of unlabeled Lox 3′UTR (wedge) were incubated with MEL S100 extract to form the Lox complex. The incubation mixtures were analyzed on native gels. The position of the uncomplexed αCP in the S100 extract and its recruitment to the position of the Lox complex were visualized by Western analysis. (C) hnRNP K was not recruited into the Lox complex. hnRNP K was detected with a monospecific antibody. The study was carried out as described above.