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. 2021 Jul;32(7):1616–1629. doi: 10.1681/ASN.2020071029

Figure 3.

Figure 3.

SGLT2 is overexpressed in the PT of nondiabetic HF rats. (A) Representative immunohistochemical staining of SGLT2 in the PT of sham and HF rats treated with empagliflozin (EMPA) or untreated. Scale bar: 50 µm. (B) Representative immunoblots from SDS-PAGE of renal cortical proteins isolated from sham and HF rats treated with EMPA or untreated and probed with antibodies against SGLT2 and β-actin. (C) Graphic representation of the relative levels of SGLT2 protein abundance in the renal cortex of the four groups of rats. (D) Graphic representation of the relative mRNA expression of SGLT2 in the renal cortex of sham and HF rats treated with EMPA or untreated. The levels of SGLT2 mRNA were measured using quantitative PCR, and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) was used as an internal control. The values represent individual measurements and the means ± SEM (Supplemental Material). *P=0.01 versus sham; **P=0.006 versus sham; ***P<0.001 versus sham; ††† P<0.001 versus sham + EMPA; ## P=0.008 versus HF; ### P<0.001 versus HF.