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. 2021 Jul 3;8(17):2101210. doi: 10.1002/advs.202101210

Figure 5.

Figure 5

B. subtilis viability and functions after freezing. a) Fluorescence field and superposed field images and of SYTO‐dyed B. subtilis in hydrogel blocks before and after freezing. b) Quantitative analysis and corresponding confocal microscopy images of B. subtilis in hydrogel blocks after thawing for 0, 2, 6, and 12 h (n = 4 for each group; Student's t‐test was conducted for comparison; ** p < 0.01, *** p < 0.001). c) Fluorescence field and superposed field images of SYTO‐dyed B. subtilis in the microneedles. The images differ in the height of z axis. d) Quantitative analysis and corresponding optical images of the inhibition rings against C. albicans (n = 5 for each group; Student's t‐test was conducted for comparison; *** p < 0.001). Scale bars: 20 µm in (a b) and 6 mm in (d).