Figure 4.

In vivo GBM tracked with ZGO@TiO₂@ALP‐NEs. a) In vivo persistent luminescence images of GL261 tumor‐bearing nude mice taken at different times post i.v. injection of ZGO@TiO₂@ALP, ZGO@TiO₂@ALP‐NEs, and ZGO@TiO₂@ALP‐NEs with ultrasound treatment (5 min, 1.5 MHz, 1.5 W cm⁻²). b) Ex vivo luminescence images of major organs and brain dissected from mice at 24 h post i.v. injection. c) Semi‐quantitative analysis of ex vivo luminescence images in different organs in (b). Data are presented as means ± s.d. (n = 3). The mice were irradiated with the LED light (650 ± 10 nm) for 2 min to activate the persistent luminescence of ZGO core before imaging in (a). The signal acquisition time was 150 s.