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General procedures of sample multiplexing for scRNA‐seq. Cells from different samples are labeled using unique sample‐specific oligonucleotides with DNA barcodes. The samples are then pooled for simultaneous library construction and sequencing. Next, sample‐specific barcodes are read alongside single‐cell transcriptomes. This is followed by in silico demultiplexing based on a predefined barcode sequence, to assign sample identity to each cell.
