Figure 6.

Critical technical challenges of barcoding technology for sample multiplexing. A) Combinatorial barcoding through multiple hybridization. B) Targeted barcoding coupled with targeted delivery system in vivo. C) Large‐scale sample multiplexing with single‐cell transcriptome profiling, alongside determination of a cellular spatial position, for establishing a complete 3‐dimensional (3D) spatial transcriptome atlas representative of many individuals. D) Sample multiplexing with multiple‐modality profiling within the same single cell to deepen our understanding of the function and composition of complex tissues, extending the range of biomedical applications.