| Gene (Mus musculus) |
H19 |
GeneBank |
NR_130973.1
|
|
| Strain, strain background (Mus musculus) |
C57BL/6 J (B6) |
Jackson Labs |
Jax: 000664 |
|
| Strain, strain background (Mus musculus) |
B6.FVB-Tg (Myh6-cre)2,182Mds/J |
Jackson Labs |
Jax: 011038 |
|
| Strain, strain background (Mus musculus) |
H19ΔICR
|
Previous study from this lab |
PMID:10817754
|
Maintained by B6 backcross |
| Strain, strain background (mouse) |
H19 BAC Transgene |
Previous study from this lab |
PMID:10921905
|
Maintained by B6 backcross |
| Strain, strain background (mouse) |
H19ΔEx1 |
Previous study from this lab |
PMID:12270940
|
Maintained by B6 backcross |
| Strain, strain background (mouse) |
H19ΔLet7 |
This study |
See Materials and methods |
Maintained by B6 backcross |
| Cell line (Rattus norvegicucs) |
H9c2 cells: Rat myocardium (embryo) |
ATCC CRL-1446 |
RRID:CVCL_0286
|
|
| Transfected construct (vector control) |
pcDNA3.1+ |
Invitrogen |
V790-20 |
|
| Biological sample (Mus musculus) |
Primary myoblasts |
This study |
See Materials and methods |
Isolated from neonates |
| Biological sample (Mus musculus) |
Primary cardiac endothelial cells |
This study |
See Materials and methods |
Freshly isolated from neonates |
| Biological sample (Mus musculus) |
Primary cardiomycytes |
This study |
See Materials and methods |
Freshly isolated from neonates |
| Antibody |
See Supplementary file 3 for list of 23 primary and six secondary antibodies |
|
|
|
| Recombinant DNA reagent |
pcDNA3-H19 |
This study |
Mouse EcoRI-SalI fragment cloned into Nhe-XhoI of pcDNA3 |
|
| Sequence-based reagent |
See Supplementary file 2 for list of oligonucleotides used for PCR |
|
|
|
| Peptide, recombinant protein |
Human Recombinant IGF2 |
Preprotech |
Catalog # 100–12 |
|
| Commercial assay or kit |
Mouse IGF2 ELISA Kit |
Abcam |
ab100696 |
|
| Commercial assay or kit |
H19 in situ probe |
Advanced Cell Diagnostics |
ACD 423751 |
|
| Commercial assay or kit |
H19 in situ probe |
Advanced Cell Diagnostics |
ACD 322360 |
|
| Chemical compound, drug |
BMS 754807 |
Active Biochem |
A-1013 |
|
| Chemical compound, drug |
MEK1/2 Inhibitor PD98059 |
Cell Signaling Technology |
#9900 |
|
| Software, algorithm |
DESeq2 |
|
RRID:SCR_015687
|
|
| Software, algorithm |
Bioconductor |
|
RRID:SCR_006442
|
|
| Software, algorithm |
Feature Counts |
|
RRID:SCR_012919
|
|
| Software, algorithm |
Debian |
|
RRID:SCR_006638
|
|
| Software, algorithm |
STAR |
|
RRID:SCR_004463
|
|
