Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M. musculus) | B6.129P2(C)-Cx3cr1tm2.1(cre/ERT2)Jung | Jackson Laboratory | IMSR Cat# JAX:020940, RRID:IMSR_JAX:020940 | |
| Strain, strain background (M. musculus) | STOCK Ascl1tm1.1(Cre/ERT2)Jejo/J | Jackson Laboratory | IMSR Cat# JAX:012882, RRID:IMSR_JAX:012882 | |
| Strain, strain background (M. musculus) | C57BL/6-Tg (Nes-cre/ERT2)KEisc/J | Jackson Laboratory | IMSR Cat# JAX:016261, RRID:IMSR_JAX:016261 | |
| Strain, strain background (M. musculus) | B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J | Jackson Laboratory | IMSR Cat# JAX:006148, RRID:IMSR_JAX:006148 | |
| Strain, strain background (M. musculus) | B6.129(Cg)-Cx3cr1tm1LittCcr2tm2.1Ifc/JernJ | Jackson Laboratory | IMSR Cat# JAX:032127, RRID:IMSR_JAX:032127 | |
| Strain, strain background (M. musculus) | B6.129S4-Ccl2tm1Rol/J | Jackson Laboratory | IMSR Cat# JAX:004434, RRID:IMSR_JAX:004434 | |
| Strain, strain background (M. musculus) | C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ | Jackson Laboratory | IMSR Cat# JAX:006567, RRID:IMSR_JAX:006567 | |
| Strain, strain background (M. musculus) | C57BL/6J | Jackson Laboratory | IMSR Cat# JAX:000664, RRID:IMSR_JAX:000664 | |
| Antibody | Anti-Iba1 (Rabbit polyclonal) | FUJIFILM Wako Shibayagi | Cat# 019–19741, RRID:AB_839504 | IF(1:1000) |
| Antibody | Anti-Iba1 (Goat polyclonal) | Abcam | Cat# ab5076, RRID:AB_2224402 | IF(1:1000) |
| Antibody | Anti-Cd11b (Rat monoclonal) | Bio-rad | Cat# MCA711, RRID:AB_321292 | IF(1:50) |
| Antibody | Anti-P2RY12 (Rabbit polyclonal) | Sigma-Aldrich | Cat# HPA014518, RRID:AB_2669027 | IF(1:200) |
| Antibody | Anti-TMEM119 (Rabbit monoclonal) | Abcam | Cat# ab209064, RRID:AB_2800343 | IF(1:200) |
| Antibody | Anti-AXL (Goat polyclonal) | R and D Systems | Cat# AF854, RRID:AB_355663 | IF(1:100) |
| Antibody | Anti-Dectin-1 (CLEC7A) (Rat monoclonal) | InvivoGen | Cat# mabg-mdect, RRID:AB_2753143 | IF(1:30) |
| Antibody | Anti-Ki67 (Rabbit monoclonal) | Abcam | Cat# ab16667, RRID:AB_302459 | IF(1:200) |
| Antibody | Anti-Ccl12 (Goat polyclonal) | R and D Systems | Cat# AF428, RRID:AB_2070875 | 100 ug/0.5 ml sterile HBSS |
| Antibody | Anti-Nestin (Mouse monoclonal) | Abcam | Cat# ab6142, RRID:AB_305313 | IF(1:200) |
| Antibody | Anti-MASH1 (Mouse monoclonal) | BD Biosciences | Cat# 556604, RRID:AB_396479 | IF(1:200) |
| Antibody | Anti-TIE2 (Mouse monoclonal) | Abcam | Cat# ab24859, RRID:AB_2255983 | IF(1:100) |
| Antibody | Anti-GFAP (Chicken polyclonal) | Abcam | Cat# ab4674, RRID:AB_304558 | IF(1:3000) |
| Antibody | Anti-doublecortin (DCX) (Goat polyclonal) | Santa Cruz Biotechnology | Cat# sc-8066, RRID:AB_2088494 | IF(1:200) |
| Antibody | Anti-Olig2 (Rabbit monoclonal) | Abcam | Cat# ab109186, RRID:AB_10861310 | IF(1:200) |
| Antibody | Anti-SOX2 (Goat polyclonal) | R and D Systems | Cat# AF2018, RRID:AB_355110 | IF(1:200) |
| Antibody | Anti-GFP (Rabbit polyclonal) | Abcam | Cat# ab6556, RRID:AB_305564 | IF(1:200) |
| Antibody | Anti-GFP (Chicken polyclonal) | Abcam | Cat# ab13970, RRID:AB_300798 | IF(1:200) |
| Antibody | Anti-PU.1 (Rabbit monoclonal) | Cell Signaling Technology | Cat# 2258, RRID:AB_2186909 | IF(1:200) |
| Antibody | Anti-Collagen IV (Rabbit polyclonal) | Abcam | Cat# ab6586, RRID:AB_305584 | IF(1:200) |
| Antibody | Anti-Parvalbumin (Mouse monoclonal) | Millipore | Cat# MAB1572, RRID:AB_2174013 | IF(1:200) |
| Antibody | Anti-Wisteria floribunda lectin (WFA) (Biotinylated) | Vector Laboratories | Cat# B-1355, RRID:AB_2336874 | IF(1:1000) |
| Antibody | Anti-PDGF Receptor alpha (PDGFRα) (Rabbit polyclonal) | Abcam | Cat# ab124392, RRID:AB_10978090 | IF(1:200) |
| Antibody | Anti-Myelin Basic Protein (MBP) (Rat monoclonal) | Millipore | Cat# MAB386, RRID:AB_94975 | IF(1:200) |
| Antibody | Anti-NeuN (Mouse monoclonal) | Millipore | Cat# MAB377, RRID:AB_2298772 | IF(1:1000) |
| Software, algorithm | Leica Application Suite X (LASX) | Leica | RRID:SCR_013673 | |
| Software, algorithm | Imaris | Bitplane | RRID:SCR_007370 | |
| Software, algorithm | Image J | Image J | RRID:SCR_003070 | |
| Software, algorithm | Fiji | Fiji | RRID:SCR_002285 | |
| Software, algorithm | Weighted Gene Co-expression Network Analysis | Software R package | RRID:SCR_003302 | |
| Software, algorithm | EdgeR | Bioconductor software package | RRID:SCR_012802 | |
| Software, algorithm | DESeq | Bioconductor software package | RRID:SCR_000154 | |
| Software, algorithm | LIMMA | Bioconductor software package | RRID:SCR_010943 | |
| Software, algorithm | Glimma | Bioconductor software package | RRID:SCR_017389 | |
| Software, algorithm | ggplot2 | Software R package | RRID:SCR_014601 | |
| Software, algorithm | EnhancedVolcano | Bioconductor software package | RRID:SCR_018931 | |
| Software, algorithm | clusterProfiler | Bioconductor software package | RRID:SCR_016884 | |
| Software, algorithm | maSigPro | Bioconductor software package | RRID:SCR_001349 | |
| Software, algorithm | Enrichr | Enrichr | RRID:SCR_001575 | |
| Software, algorithm | Ethovision XT | Noldus | RRID:SCR_000441 | |
| Software, algorithm | GraphPad Prism | GraphPad | RRID:SCR_002798 |