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. 2021 Sep 1;2021:2202888. doi: 10.1155/2021/2202888

Figure 5.

Figure 5

miR-137 inhibited hypoxia-induced upregulation of calpain-2 expression. (a) The putative binding site of miR-137 in 3′-UTR of calapin-2 mRNA. (b) Luciferase analysis for examining whether miR-137 targets 3′-UTR of calpain-2 mRNA (n = 3). (c) The mRNA expression of calpain-2 in PASMCs after transfecting miR-137 mimic under normoxic condition (n = 5). (d) The protein expression of calpain-2 in PASMCs after transfecting miR-137 mimic (25 nM) under normoxic condition (n = 3). (e) The mRNA expression of calpain-2 in PASMCs after transfecting miR-137 mimic (25 nM) under hypoxic condition (n = 4). (f) The protein expression of calpain-2 in PASMCs after transfecting miR-137 mimic (25 nM) under hypoxic condition (n = 4). WT: wild type; Mut: mutant; NC: negative control. The data are presented as means ± S.E.M.; ∗p < 0.05 and ∗∗p < 0.01 vs. control or WT+NC control and ##p < 0.01 vs. hypoxia.