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. Author manuscript; available in PMC: 2021 Sep 8.
Published in final edited form as: ACS Chem Neurosci. 2021 Apr 12;12(8):1428–1437. doi: 10.1021/acschemneuro.1c00094

Figure 1.

Figure 1.

Human liver microsome stability assay of MCL-536. Briefly, MCL-536 was preincubated with pooled human microsomes in 100 mM phosphate buffer; then NAPDH (final concentration 1 mM) was added. After 30 or 60 min incubation, the reaction was stopped, and samples were analyzed with a Varian Prostar HPLC system on Agilent Microsorb-MV 100 C18 columns fitted with a Microsorb 100-5 C18 MetaGuard column and a detector set at 265 nm. Dextromethorphan (DXM) was used as positive control (detection at 278 nm); negative controls included DXM incubated with heat-inactivated microsomes (HDXM) and one incubation mix without NADPH. The experiment was performed in triplicate. (A) Structure of MCL 536. (B) Microsome stability assay.