FIG. 4.

Coimmunoprecipitation of endogenous BRCA2 with DSS1 in MCF7 cells. Lysates of MCF7 cells cultured below confluency in 10% fetal calf serum, and lysates of COS cells transiently transfected with (+) or without (−) a construct expressing full-length (F.L.) BRCA2 were immunoprecipitated, in parallel, with anti-BRCA2 antiserum B, anti-DSS1 antiserum 290, or their preimmune sera (B PI and 290 PI). Immunoprecipitates were analyzed by SDS-PAGE (6% polyacrylamide gel) and Western blotting with an anti-BRCA2 rat monoclonal antibody.