4–12% NuPAGE gel (Invitrogen, Carlsbad, CA) loaded with 1μg each of insect cell expressed, immunoaffinity purified recombinant filovirus proteins. Lane M: Prestained molecular weight marker (Seeblue Plus2, Invitrogen); Lane 1: EBOV GP, Lane 2: MARV GP, Lane 3 SUDV GP, Lane 4: EBOV VP24, lane 5: EBOV VP40. SUDV and MARV GP proteins are expressed using an analogous process to EBOV proteins and are shown here for reference.