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. Author manuscript; available in PMC: 2021 Sep 8.
Published in final edited form as: Vaccine. 2017 Feb 16;36(22):3090–3100. doi: 10.1016/j.vaccine.2017.01.068

Figure 4. Cell-mediated immune responses raised by recombinant antigens.

Figure 4.

Panel A: Mean (n=5 per group) lymphocyte proliferation (indicated as stimulation index, SI) in vitro from immune splenocytes stimulated with homologous antigens. Mean SI from mitogen (PHA) stimulated cultures varied in the range of 4.2–42. Mean SI in splenocyte cultures from adjuvant only immunized mice re-stimulated with GP, VP24, or VP40 was <2.0 in all cases.

Panel B: Mean (n=5 per group) IFN-γ production in vitro from immune splenocytes re-stimulated with homologous antigens. Mean IFN-γ production from PWM stimulated cultures varied in the range of 15–58 ng/mL. Mean IFN-γ production from control (unstimulated) cultures was <0.5 ng/mL in all cases. Mean IFN-γ production in splenocyte cultures from adjuvant only immunized mice re-stimulated with GP, VP24, or VP40 was <1.0 ng/mL in all cases.

Panel C: Mean (n=5 per group) IL-4 production in vitro from immune splenocytes re-stimulated with homologous antigens. Mean IL-4 production from PWM stimulated cultures varied in the range of 5.7–11.7 ng/mL. Mean IL-4 production from control (unstimulated) cultures was <0.25 ng/mL in all cases. Mean IL-4 production in splenocyte cultures from adjuvant only immunized mice stimulated with GP, VP24, or VP40 was <0.2 ng/mL in all cases. Differences in IL-4 production between groups immunized with formulations containing GPI-0100 or ISA51 were significant (p < 0.05) between the groups immunized and re-stimulated using the same antigen. Differences in IFN-γ production or proliferation were not significant (p > 0.05) for formulations using the two different adjuvants suggesting that adjuvant has a lower effect on Th1 type responses.