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IL-1-induced activation of Jun kinase in mutant cells. Immunoprecipitates were prepared from cell extracts with anti-Jun kinase, followed by an in vitro kinase assay. (A) Analysis of extracts from 293-TK/Zeo (WT [wild type]) cells and I1A cells, untransfected or stably transfected with IRAK or IRAK-K239A, untreated, stimulated with IL-1, or treated with UV (40 J/m2). (B) Analysis of extracts from clones I1A, I2A, and I3A untreated, stimulated with IL-1, or treated with UV (40 J/m2).
