Fig. 1.

Expression pattern of E2A and key markers regulating in vitro CMs directed differentiation.

(a) Directed differentiation of hESCs into CMs by modulating Wnt signaling activity. (b) Line graphs depicting the mRNA levels of key markers at different differentiation stages of cardiomyocytes differentiation. (c) The increased ratios of MYH7/MYH6 and MYL2/MYL7 demonstrated the maturation of cardiomyocytes over prolonged culture. (d) mRNA levels of E2A during CMs differentiation process. All data were normalized to the values on day 0 and presented as mean ± SEM (n=3).