Table 1.
H7-WT&H7-KO single cell Patch-Clamp recording Statistics
| MDP (mV) | Overshoot (mV) | APA (mV) | APD (ms) | APD90 (ms) | APD70 (ms) | APD50 (ms) | Vmax-D (V/s) | Beating rates (per minute) | SD of Interspike Interval | % Total | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ventricular-like (V-like) CMs | WT(n=28) | -56.7±0.8 | 45.9±0.8 | 102.7±1.0 | 358.3±25.8 | 290.1±22.9 | 270.0±21.9 | 251.4±20.3 | 8.0±0.4 | 87.7±7.0 | 45.2±7.6 | 75.7 |
| KO(n=18) | -59.7±0.8 | 42.6±1.2 | 102.2±1.4 | 340.1±33.9 | 256.6±30.0 | 238.8±28.9 | 220.4±27.4 | 9.8±0.5 | 95.5±8.2 | 37.3±7.5 | 39.1 | |
| Atrial-like (A-like) CMs | WT(n=8) | -56.0±1.9 | 38.8±1.7 | 94.8±1.1 | 282.6±49.5 | 219.4±39.5 | 198.0±34.8 | 175.1±28.7 | 5.7±0.3 | 121.0±12.2 | 19.5±2.2 | 21.6 |
| KO(n=9) | -58.6±1.4 | 35.5±1.0 | 94.1±1.5 | 218.8±16.2 | 142.7±9.0 | 125.3±7.7 | 110.2±6.5 | 8.2±1.1 | 118.9±12.3 | 27.6±12.1 | 19.6 | |
| Nodal-like (N-like) CMs | WT(n=1) | -46.1 | 27.5 | 73.6 | 143 | 89.5 | 75.1 | 63.7 | 3.6 | 101.5 | 54.2 | 2.7 |
| KO(n=19) | -54.3±1.2 | 27.4±1.4 | 81.7±1.9 | 192.2±10.7 | 101.7±5.9 | 81.7±5.2 | 67.4±4.4 | 6.8±0.9 | 130.2±8.5 | 22.9±3.9 | 41.3 |