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. 2021 Feb 19;48(10):3089–3100. doi: 10.1007/s00259-021-05243-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5a — Intravital microscopy image of TRITC-dextran filled lymphatics (white) superimposed on transillumination image of blood vessels (black). The image shows an A-07-GFP melanoma xenograft (delineated by red line) and the surrounding normal tissue. Images of lymphatics were recorded 10–15 min after 3–5 intradermal injections of ~2 μl TRITC-dextran in the skin surrounding the window chambers. b Lymphatic diameter versus time for window chambers with and without A-07-GFP tumors. Points, means of 8–13 window chambers; bars, standard error. c Change in lymphatic diameter versus tumor size. Points, individual window chambers; line, curve fitted to the data by linear regression. Fig. 5b-c was modified from Gaustad et al. [45]