Fig. 5.

Analysis of mRNA expression by RT-qPCR in MEFs and brain tissue of 3- and 11–19-month-old mice, focusing on a induced interferon-stimulated genes, b cytosolic RNA sensors, c cytosolic DNA sensors, d RLRs with associated factors, e TLRs with associated factors, and f NLRs. RT-qPCRs were normalized to Tbp levels. Data are presented as mean ± SEM. WTs are shown as checked, and ClpP^−/− as plain colored bar graphs. Statistical tendencies and significances are illustrated by symbols: * or #/§ p < 0.05; ** or ##/§§ p < 0.01; *** or ###/§§§ p < 0.001; **** or §§§§ p < 0.0001; not significant (ns) p > 0.05; tendency (T) 0.05 < p < 0.1. Asterisks portray significant differences between WT and ClpP^−/− MEF, hashtags illustrate significant effects in brain tissue between 3-month-old WT and ClpP^−/− mice, and section signs visualize significant differences in brain of 11–19-month-old WT versus ClpP^−/− mice. WT MEF: n= 3–9; ClpP^−/− MEF: n= 3–8; WT brain 3 months: n= 3; ClpP^−/− brain 3 months: n= 3; WT brain 11–19 months: n= 5; ClpP^−/− brain 11–19 months: n= 5–6