REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Ultra-LEAF™ Purified anti-human CD3 (clone UCHT1)	BioLegend	Cat#300465
Ultra-LEAF™ Purified anti-human CD28 (clone CD28.2)	BioLegend	Cat#302934
Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl (clone MG1-45)	BioLegend	Cat#401407
APC/Cyanine7 anti-human CD3 (clone HIT3A)	BioLegend	Cat#300318
Alexa Fluor® 700 anti-human CD4 (clone RPA-T4)	BioLegend	Cat#300526
PE/Cyanine7 anti-human CD8a (clone RPA-T8)	BioLegend	Cat#301012
Brilliant Violet 605™ anti-human CD45RA (clone HI100)	BioLegend	Cat#304134
Alexa Fluor® 488 anti-human CD197 (CCR7) (clone G043H7)	BioLegend	Cat#353206
PE/Dazzle™ 594 anti-human CD197 (CCR7) (clone G043H7)	BioLegend	Cat#353236
PerCP/Cyanine5.5 anti-human CD19 (clone HIB19)	BioLegend	Cat#302230
Biological Samples
Whole blood of healthy donors	New York Blood Center	N/A
Chemicals, Peptides, and Recombinant Proteins
Recombinant Human B7-H1 (PD-L1, CD274)-Fc Chimera (carrier-free)	BioLegend	Cat#762506
Recombinant Human PD-L2 (B7-DC, CD273)-Fc Chimera (carrier-free)	BioLegend	Cat#772508
Staphylococcus Enterotoxin E (SEE)	Toxin Technology	Cat#ET404
Lymphoprep density gradient	STEMCELL Technologies	Cat#07851
RosetteSep™ Human T Cell Enrichment Cocktail	STEMCELL Technologies	Cat#15061
CFSE Cell Division Tracker Kit	BioLegend	Cat#423801
CellTrace™ Far Red Cell Proliferation Kit	Thermo Fisher Scientific	Cat#C34572
Zombie UV™ Fixable Viability Kit	BioLegend	Cat#423108
CD8 MicroBeads, human	Miltenyi Biotec	Cat#130-045-201
Naive CD4+ T Cell Isolation Kit II, human	Miltenyi Biotec	Cat#130-094-131
Memory CD4+ T Cell Isolation Kit, human	Miltenyi Biotec	Cat#130-091-893
Deposited Data
bulk RNAseq data	This manuscript	GEO: GSE174860
Single-cell RNAseq data	https://doi.org/10.1016/j.cell.2018.10.038	GEO: GSE120575
Experimental Models: Cell Lines
Raji (ATCC® CCL-86™)	ATCC	ATCC® CCL-86™
Recombinant DNA
pHR-PD-L1-mCherry	Hui et al.Science. 2017 Mar 31; 355(6332):1428-1433	N/A
pHR-PD-L2-mCherry	Philips et al.J Biol Chem. 2020 Apr 3; 295(14): 4372–4380	N/A
pHR-control	This manuscript	N/A
Software and Algorithms
bulk RNAseq data analysis	https://app.basepairtech.com/	N/A
Gene ontology enrichment analysis	http://www.pantherdb.org	N/A
Transcription factor enrichment analysis	https://maayanlab.cloud/chea3	N/A
Single cell RNAseq analysis	https://bioturing.com	N/A
Survival analysis	kmplot.com	N/A
Gene expression associations in TCGA	http://timer.cistrome.org	N/A
GraphPad Prism 9	https://www.graphpad.com	N/A
FlowJo 10.1r7	https://www.flowjo.com	N/A