Fig. 5.

Comparison of cell membrane N-glycome obtained from the cortex, hippocampus, and cerebellum. Relative abundances of N-glycan groups in the cerebral cortex, hippocampus, and cerebellum (A), and total levels of fucosylation and sialylation (B). Asterisks indicate the statistical significance between groups compared (∗p < 0.05%; ∗∗p < 0.01%; ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001). Heatmap of N-glycans differentially expressed in the cortex, hippocampus and cerebellum, ordered from high mannose to complex glycans approximately according to N-glycan biosynthesis (p < 0.05%, as analyzed by two-way ANOVA and the TUKEY post-hoc test) (C). Glycan mass of hybrid and complex glycans increases from top to bottom. Glycan composition is abbreviated as Hex_HexNAc_Fuc_NeuAc where Hex = Hexose, HexNAc = N-Acetylhexosamine, Fuc = Fucose, NeuAc = N-Acetylneuraminic Acid; and numbers indicate the number of each monosaccharide residue. Abundance for each glycan composition was normalized to the abundance of total glycans identified. Data from six biological replicates were used. Partial least squares–discriminant analysis (PLS-DA) scores plots for cell membrane N-glycan profiles of the cortex, hippocampus, and cerebellum (D), and cortex and hippocampus (F). The 95% confidence interval is indicated as an ellipse, and each symbol represents a mouse (n = 6). The loadings plots (E and G) from the PLS-DA model show glycans contributing to the separation in the first dimension (x-axis of the scores plot) for cortex, hippocampus, and cerebellum (E), and cortex and hippocampus (G). C, complex; H, hybrid.