Fig. 1.

Computed tomography is able to detect advanced BLM-induced lung fibrosis. a Representative lung CT images of NaCl- and BLM-receiving mice at D21. Graph represents the mean lung density quantified on CT images. Results are presented as mean ± SEM, n = 3 for NaCl and n = 6 for BLM. *p < 0.05. HU, Hounsfield unit. b Representative 3D reconstruction of lung 3DROI segmentation of lung CT images of NaCl- and BLM-receiving mice at D21. Red represents high-density lung areas (− 100 to 300 HU) representative of non-aerated lungs; grey represents normal density lung areas (− 800 to − 100 HU) representative of aerated lungs. Graph represents the percentage of aerated lung on CT images. Results are presented as mean ± SEM, n = 3 for NaCl and n = 6 for BLM, *p < 0.05. c Representative Picrosirius red staining lung sections of NaCl- and BLM-receiving mice at D21. Graph represents the intensity of picrosirius red staining. Results are presented as mean ± SEM, n = 3 for NaCl and n = 6 for BLM, *p < 0.05. d Correlation between mean lung density (HU) measured on CT images and picrosirius red staining of corresponding lungs at D21