Fig. 3.

[¹⁸F]FMISO is able to detect advanced BLM-induced lung fibrosis. a Representative lung PET/CT images with [¹⁸F]FMISO of NaCl- and BLM-receiving mice at D21. Graph represents the [¹⁸F]FMISO lung uptake in %ID/g of NaCl- and BLM-receiving mice at D21. Results are presented as mean ± SEM, n = 3 for NaCl and n = 6 for BLM. *p < 0.05. b Graph represents the [¹⁸F]FMISO lung uptake in %ID/g of NaCl- and BLM-receiving mice at D21 in aerated and non-aerated lung areas (segmented on CT images). Results are presented as mean ± SEM, n = 3 for NaCl and n = 6 for BLM. c Correlation between mean lung density (HU) measured on CT images and [¹⁸F]FMISO lung uptake (%ID/g) of corresponding lungs measured on PET at D21. d Representative 3D reconstruction of lung 3DROI segmentation of lung PET images representative of hypoxic lung volume (HLV) of NaCl- and BLM-receiving mice at D21. Cyan represents areas with high [¹⁸F]FMISO lung uptake (above the threshold of HLV_threshold = TBR × 1.4); grey represents areas with low [¹⁸F]FMISO lung uptake (below the threshold). Graph represents the MLV of NaCl- and BLM-receiving mice at D21. Results are presented as mean ± SEM, n = 3 for NaCl and n = 6 for BLM. *p < 0.05. e Correlation between mean lung density (HU) measured on CT images and HLV (%) of corresponding lungs measured on PET at D21. f Radioactivity content in lungs of NaCl- and BLM-receiving mice and lung to blood ratio at D21 measured ex vivo with a γ-counter. Results are presented as mean ± SEM, n = 3 for NaCl and n = 6 for BLM. *p < 0.05