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. 2021 Sep 9;40(42):6130–6138. doi: 10.1038/s41388-021-02005-y

Fig. 5. Intron retention, spliceosome activity, and MHC abundance in MM cells.

Fig. 5

A Number of intron retention events determined from CCLE RNA-seq data in a panel of four MM cell lines. B MM cell lines with high IR levels had lower baseline MHC-II cell surface expression determined by flow cytometry. C HLA class I gene score (average expression of HLA-A/B/C alleles) from MMRF RNA-seq data was negatively associated with spliceosome pathway activity. D MHC-I genes were upregulated in a dose-dependent manner in three of four MM cell lines following treatment with the splicing inhibitor pladienolide-B (0–100 nM) for 96 h. E Representative flow cytometry plots illustrating the percentage of KMS11 cells with increased MHC-I (HLA-A/B/C) gene expression following treatment with pladienolide-B (0–100 nM) for 96 h. Gates were set based on isotype controls and unstained controls.