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. 2021 Sep 8;11:17827. doi: 10.1038/s41598-021-97293-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evolution of carbamylation in bovine aorta with age. Homocitrulline (HCit) was quantified by LC–MS/MS in (A) total aorta extracts and in (B) aorta elastin of 21 bovine samples. Nonlinear regressions (95% confidence interval; shaded zones) and linear regressions (solid lines) of HCit concentrations over time were calculated. Spearman coefficients of correlation (r) showed a significant HCit increase with age (p < 0.05 for total extracts and p < 0.001 for elastin).