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. 2021 Sep 8;11:17827. doi: 10.1038/s41598-021-97293-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Properties of aortic elastic fibers after carbamylation enhancement in mice. (A) HCit was quantified by LC–MS/MS in total aorta extracts in WT mice drinking water supplemented with 1 mM NaCl (control) or 1 mM NaCNO (NaCNO) for 3 weeks. Results were expressed in mmol HCit per mol Lys. (B) Representative confocal images of aortic elastic fibers (using autofluorescence properties of elastin). L vessel lumen. (C) Elastic fiber degradation rates were expressed as rupture index corresponding to the ratio of the number of breaking points to the total area of elastic fibers. For (A) and (C), results were presented as boxplots, in which the error bars represent minimum and maximum values, the horizontal bars and the crosses indicate median and mean values, respectively, and the extremities of the boxes indicate interquartile ranges. Values were compared using the non-parametric Mann–Whitney's U test (ns not significant, **p < 0.01). a.u. arbitrary units.