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. 2021 Sep 8;11:17828. doi: 10.1038/s41598-021-96877-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Spectrographic flow measurements (healthy subject). (a,b) Blood flow and directional blood flow. (c) Color composite image of systolodiastolic blood flow changes. (d) Spectrograms in an in-plane artery (‘A’) where multiply scattered light contributes to the Doppler spectrum, and in the emergence of the central retinal artery (‘CRA’) and central retinal vein (‘CRV’) where backscattered light is the main contribution the Doppler spectrum. (e) Symmetrized spectrograms in the same regions. (f) The spectrogram in the region ‘T’ shows the ONH tissue motion. (g) Baseline spectrogram (spatial average). Visualization 1 shows the directional blood flow movie juxtaposed to the spectrogram in the ‘CRA’ region. The estimated size of the scale bar is 500 $μ$ m.