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. 2021 Sep 8;7:68. doi: 10.1186/s40981-021-00472-6

Table 1.

Results of skin tests

Skin prick test Intradermal test
Drug Concentration of the stock solution (mg/mL) Judgment Wheal (mm) Flare (mm) Concentration of the stock solution (mg/mL) Judgment Wheal (mm) Flare (mm)
Saline 9 9
Histamine 10 + 5 10 0.01 + 9 20
Propofol 10 1
Rocuronium 10 0.1
Remifentanil 0.05 0.005
Fentanyl 0.05 0.005
Cefazolin 20 2
Flurbiprofen 10 1
Lidocaine 10 1
Ropivacaine 2 0.2
Felt NA NA ND
Vial no. 1 80 8
Vial no. 2 0.17 + 5 6 0.017 + 7 12
Vial no. 3 250a 25a

aUnits/mL

SPT skin prick test, IDT intradermal test, NA not applicable, ND no data

Saline and histamine were used as negative and positive controls, respectively. SPT was started with a 100-fold dilution of stock solution, and a tenfold dilution and stock solution were inspected. The SPTs for the felt were performed by puncturing a bifurcated needle from above the felt. Histamine and vial no. 2 showed a positive result at the stock solution. Subsequently, IDTs were performed. After confirming a negative reaction for saline and a positive reaction for histamine, vial no. 2 showed a positive reaction at a 100-fold dilution of the stock solution, that is, 0.00017 mg/ml. Other tested agents did not result in a positive reaction with a 100-fold dilution of the stock solution. SPT reactions were deemed positive when the diameter of the wheal was equal to or 3 mm larger than in the negative control, or equal to at least half that in the positive control after 20 min. IDT reactions were deemed positive when the diameter of the wheal was equal to at least twice that of the post-injection wheal after 20 min [6]