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. Author manuscript; available in PMC: 2022 Apr 2.
Published in final edited form as: J Mol Biol. 2021 Jan 13;433(7):166805. doi: 10.1016/j.jmb.2020.166805

Table 3.

Number of chromosomal sites in common in each dataset pair and their statistical consitency in permutation tests.

Feature of dataset 1 Feature of dataset 2 Random permutations features
Cell line 1 Transposon 1 Transposase 1 Cell line 2 Transposon 2 Transposase 2 Window around insertion sites (±) in both datasets Number of sites observed Average number of sites expected per chance Standard deviation Z score Probability = H0 was no differences between obs. and exp.
HeLa Ifp2 PB HeLa Ifp2 PB.NLS-1–558 0 1 N.A. N.A. N.A. p<0.001
HeLa Ifp2 PB HeLa Ifp2 PB.NLS-1–558 1000 37 N.A. N.A. N.A. p<0.001
HeLa Ifp2 PB HeLa Ifp2 Mm523-PGBD5 0 83 N.A. N.A. N.A. p<0.001
HeLa Ifp2 PB HeLa Ifp2 Mm523-PGBD5 1000 175 N.A. N.A. N.A. p<0.001
HeLa Ifp2 PB G401 Ifp2 Hs524-PGBD5 0 24 N.A. N.A. N.A. p<0.001
HeLa Ifp2 PB G401 Ifp2 Hs524-PGBD5 1000 42 N.A. N.A. N.A. p<0.001
HeLa Ifp2 PB HEK293 Ifp2 PB 0 21 N.A. N.A. N.A. p<0.001
HeLa Ifp2 PB HEK293 Ifp2 PB 1000 471 260.162 17.0215 12.3865 0
HeLa Ifp2 PB HCT116 Ifp2 PB 0 238 67.01 8.4329 20.2763 0
HeLa Ifp2 PB HCT116 Ifp2 PB 1000 2387 N.A. N.A. N.A. p<0.006
HeLa Ifp2 PB CD4+ Ifp2 PB 0 24 N.A. N.A. N.A. p<0.001
HeLa Ifp2 PB CD4- Ifp2 PB 1000 213 97.684 10.2987 11,197 0
HEK293 Ifp2 PB HCT116 Ifp2 PB 0 391 N.A. N.A. N.A. p<0.001
HEK293 Ifp2 PB HCT116 Ifp2 PB 1000 6600 5419.484 93.3269 12.0060 0
HEK293 Ifp2 PB CD4+ Ifp2 PB 0 362 N.A. N.A. N.A. p<0.001
HEK293 Ifp2 PB CD4+ Ifp2 PB 1000 752 N.A. N.A. N.A. p<0.001
HCT116 Ifp2 PB CD4+ Ifp2 PB 0 16 81.825 9.0919 17.5073 0
HCT116 Ifp2 PB CD4+ Ifp2 PB 1000 5235 2213.501 50.9891 59.2576 0
HEK293 Sleeping Beauty SB CD4+ Sleeping Beauty SB 0 3 N.A. N.A. N.A. p>0.083
HEK293 Sleeping Beauty SB CD4+ Sleeping Beauty SB 1000 385 349.967 19.09 1.8350 p=0.034

N.A., not appropriated to use a Z-test as the distribution of the 1000 permutations results did not fulfil normality in a Shapiro-Wilk test. In the most right column is typed in red probabilities supporting that there was no difference at a threshold of 0.01.