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. 2021 Sep 8;11:17857. doi: 10.1038/s41598-021-97437-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Level of A. salmonicida subsp. salmonicida gene (aopO) transcripts in different organs of fish at 3 days post challenge (dpc): NCS: exposed fish with no clinical signs, CS: exposed fish with clinical signs. Fifteen fish from each group were investigated. Number above the square brackets indicate p values between groups. For the NCS group, only few fish showed positive Cq values, therefore qualitative assessment was applied (presence or absence of Cq values) and data was analyzed with nonparametric Mann–Whitney test.