Fig. 6. MitoQ ameliorates CISD3 associated ferroptosis via regulation mitophagy and the process of mitochondrial dynamics.

A Cells were transfected with LC3-GFP, after indicated treatment (7.5 µM erastin; 0.5 µM mitoQ) for 4 h, cells were co-stained with Mitotracker. The images were taken by a laser confocal microscope. Scale bar: 10 μm. B, C Protein levels were analyzed by western blot in control and shCISD3 cells with the designed treatment. β-actin served as the loading control. D Cell viability was assayed using CCK8 kit in the indicated cells with or without 7.5 µM erastin treatment for 12 h. E Lipid peroxides were assayed by BODIPY staining in the indicated cells with or without 7.5 µM erastin treatment. *P < 0.05, **P < 0.01 versus control or between different groups.