Fig. 5.
Results of simulation of collagen detection using multi-wavelength photoacoustic (MWPA) analysis. (a–c) PA physio-chemical spectra (PAPCS) of bone specimens with different BV/TVs. (d) Normalized optical absorption coefficient μa versus wavelength for collagen, lipid, and water. (e) Comparison of simulated PA spectra with fitted spectra obtained using the least-squares method for the model bone specimens with different BV/TVs. (f) Analysis of correlation between the relative collagen content as obtained via MWPA and the positive-stained area metric for the collagen content extracted from the histological images.