Figure 5.

Innexin2 is expressed in the small and large ventral lateral neurons in the circadian pacemaker circuit

Representative images of Drosophila adult brains showing the distribution of INX2 protein among clock cells. Flies were dissected at ZT12 and stained with anti-INX2 antibody and co-stained with anti-PDF for identification and co-localization with ventral lateral neurons. INX2 was found to be predominantly localized to the small and large ventral lateral neurons (s-LNv and l-LNv) among the clock neurons (arrows, top panel). INX2 was also found to be present in the varicosities of l-LNv projections in the optic lobes (arrowheads, bottom panel). Brightness and contrast of representative images were adjusted to facilitate better visualization. Arrows are used to indicate s-LNvs and l-LNvs. Scale-bar represents 55 μm, n = 12 brain samples were imaged. See also Figure S6.