Figure 1.
AAV Dose-Response. Mice were injected with PHP.eB-CAG-GFP or saline and tissue was collected 4 weeks later. (A) Graph of percent area staining of GFP. Data points represent values for each mouse; mean ± SEM (n = 3 for 5 × 109 vg; n = 4 for remaining groups) are indicated. **** denotes p < 0.0001 by one-way ANOVA and Tukey's post-hoc test. Bars annotated with the same lower-case letter are not statistically different from each other. (B) Representative images with anti-GFP staining. Rectangles on the CTX panels indicate area enlarged on the right (20 × panels). Arrowheads indicate presumptive glial cells. CTX and HPC scale bar = 500 μm, 20 × scale bar = 50 μm. vg, viral genomes; CTX, cortex; HPC, hippocampus.