Figure 8.

Dose-corrected γ-H2AX pS139/53BP1 foci levels in quiescent G0/G1-phase normal NFF28 primary fibroblasts pretreated for 18 h with 0.1% DMSO (vehicle control) or 10 µM SAHA measured by immunocytochemistry/image analysis in samples fixed 10 min to 24 h post-irradiation following low dose (5–25 cGy) exposures of cesium-137 γ-rays (A), 200 MeV protons (B), and 290 MeV/n C-12 ions (C). Peak foci formation occurs at 30 min post-IR for all three radiation qualities. Data reported as IR-induced means ± SD, background-subtracted for foci levels measured in sham-irradiated cultures (indicated by dashed line). Asterisks mark significant differences compared to DMSO controls at p-values of ≤0.05 (*) by two-tailed Student’s t-tests.