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SAMHD1 T592E is not oxidized in cells. PC3 and HEK293 cells expressing HA-SAMHD1 were treated with the growth factor, LPA, over time and harvested in the presence of DCP-Bio1 to capture proteins with cysteine sulfenic acids. Western blots of pulldowns probed with α-HA antibodies show oxidized SAMHD1 in cells expressing wild-type or T592A variant. No oxidized SAMHD1 appears in cells expressing the T952E or oxidation insensitive C522A variants.
