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. 2021 Aug 26;8:726731. doi: 10.3389/fvets.2021.726731

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Copyright © 2021 Agwunobi, Zhang, Shi, Zhang, Zhang, Wang, Masoudi, Yu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects on the cold tolerance of ticks after the knockdown of Dnmts. (A) The mortality rate of D. silvarum after cold treatment post DsDnmt and DsDnmt1 knockdown. The mortality rate increased significantly in the ticks with Dnmts' knockdown in relation to the controls. (B) The mortality rate of H. longicornis after cold treatment post HlDnmt1 and HlDnmt knockdown H. longicornis. The mortality rate increased significantly in the ticks with Dnmts' knockdown in relation to the controls. The results are expressed as the means (n = 3) ± SEM. The asterisks above bars indicate significant difference based on one-way ANOVA followed by Turkey's test, *P < 0.05, ***P < 0.01.