Table 1.
Primer sequences used for cDNA cloning, real-time quantitative PCR, and dsRNA synthesis.
| Tick species | Gene name | Primer sequence (5′-3′) |
|---|---|---|
| Primers of DNA methyltransferase genes for PCR | ||
| Dermacentor silvarum | Dnmt | F1:CTCGCAGAGTGCTCAACAGG |
| R1:GGCTTGTTCTTCAGCGTGG | ||
| F2:GTGAACACCACGGCGAACT | ||
| R2:TCACTGAGGCTGAGGAAACTTG | ||
| F3:TGGCTACCACATTCATCGTTA | ||
| R3:GCAGCATTTCCAGGACATCT | ||
| F4:TCAAGTTTCCTCAGCCTCAGTG | ||
| R4:GATGAAATAAGGTCCCCGAGAG | ||
| Dnmt1 | F:GGTCGGACGAGAGGTTTTTC | |
| R:GGGAAACCTGGGCAAAACT | ||
| Haemaphysalis longicornis | Dnmt1 | F1:GTCGTTTCCGCACTTCGTAG |
| R1:GTTGAACTTGGCAAAAGGGTAG | ||
| F2:TCAAGCGACCTGAAGGAATG | ||
| R2:TTCATCCTATGGCTCCGTAAAG | ||
| F3:GGTTCCTGATTGTGCGAGA | ||
| R3:TGTTCAATGGCCTTCGTCT | ||
| F4:GCAGAAGAGCAGCCGCAT | ||
| R4:CTCCTTGTTTCTGAACTGCTCG | ||
| Dnmt | F1:AGTGTGTTCAACGACGGGG | |
| R1:TCGATCACCAGCTTGCTCAT | ||
| F2:TATCGACGCCATCAAGGAGA | ||
| R2:GCCGCCAAACTTCACCAT | ||
| F3:AAGCGTTGCGGAGTCTGTG | ||
| R3:GTGCCCCTCCAAAACCAGT | ||
| F4:GACCCTGGAACCCCACTCTAT | ||
| R4:CCGCTCTCTTCTTCGACAACT | ||
| Primers for Real-time quantitative PCR | ||
| Dermacentor silvarum | Dnmt | F:TGTCAAGTTTCCTCAGCCTCA |
| R:CGACATAATGACCATAGCCCTT | ||
| Dnmt1 | F:AAGAACTCGCTGGTGTCATCGT | |
| R:TCCAGAGTCAGCGGCAACAT | ||
| Actin | F:TTCCAGCCCTCGTTCCTGGGTAT | |
| R:AATGATCTTGATCTTCATGGT | ||
| Haemaphysalis longicornis | Dnmt1 | F:GTGGCTGATGAAGGCAAAGA |
| R:CGGCAGAGTTCAAGCAGGT | ||
| Dnmt | F:TCGTCAATGAATGCGAGAACC | |
| R:AGAACGACTTGCCGTCATCATC | ||
| Actin | F:CGTTCCTGGGTATGGAATCG | |
| R:TCCACGTCGCACTTCATGAT | ||
| dsRNA synthesis primers | ||
| Dermacentor silvarum | Dnmt | F:GCGTTCAACTTCCCCGACA |
| R:GCCAGGCAGTAGAAACGCATT | ||
| Dnmt1 | F:GCGATGTGCTAAAGGGAAAGA | |
| R:CTGAAGCGAGGCAAAGGTG | ||
| Dnmt1 | F:CAACACCCCTGAAGTTAGCAA | |
| R:TCAGCCACTCTTCGCCAA | ||
| Dnmt | F:TCGCCAAGTTCACGGAGGA | |
| R:TGCCGCCAAACTTCACCAT | ||
Oligonucleotide primers for the synthesis of dsRNA comprised of T7 promoter sequences at the 5′ end.