Table 1.
0 | 0–3 h | D1 | D2 | D3 | D4 | D5 | D12 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CT | Sample | B | R | B | R | B | R | B | R | B | R | B | R | B | R | |
<25 | 1 | 21.98 | 21.1 | 21.76 | 22.56 | 23.09 | 21.98 | 22.59 | 22.8 | 24.31 | 22.84 | 22.07 | 23.8 | 26.01 | 25.4 | |
2 | 22.1 | 17.94 | 21.36 | 21.36 | 23.02 | 23.52 | 23.94 | 22.66 | 26.21 | 25.67 | 24.27 | 26.79 | 27.89 | 28.7 | ||
3 | 22.25 | 19.94 | 23.45 | 21.8 | 24.73 | 20.75 | 24.04 | 22.06 | 26.07 | 24.14 | 24.9 | 25.56 | 30.44 | 28.55 | ||
4 | 22.59 | 21.73 | 22.37 | 22.72 | 23.11 | 23.21 | 33.01 | 24.22 | 25.44 | 25.23 | 25.03 | 26.05 | 29.13 | 28.84 | ||
5 | 24.38 | 23.23 | 24.58 | 25 | 25.08 | 24.73 | 26.12 | 25.06 | 26.52 | 26.93 | 25.76 | 27.29 | 30.25 | 29.97 | ||
25–32 | 6 | 25.58 | 20.09 | 21.25 | 21.72 | 22.06 | 22.41 | 23.63 | 23.67 | 25.95 | 26.44 | 26.37 | 28.86 | 30.43 | 30.4 | |
Room | 7 | 28.76 | 26.71 | 28.51 | 28.46 | 28.31 | 28.16 | 28.79 | 27.78 | 29.88 | 29.37 | 27.92 | 30.98 | – | – | |
Temperature | 8 | 29.3 | 26.81 | 29.92 | 27.74 | 30.72 | 28.66 | 28.04 | 28 | 31.31 | 31.04 | 28.52 | 31.71 | – | – | |
9 | 29.05 | 27.67 | 27.83 | 28.35 | 28.01 | 27.56 | 28.39 | 28.66 | 28.16 | 28.7 | 27.33 | 28.86 | 34 | 34.62 | ||
10 | 29.38 | 27.62 | 26.99 | 28.56 | 27.39 | 27.94 | 27.94 | 29 | 29.32 | 29.88 | 29.23 | 29.57 | – | – | ||
11 | 30.76 | 30.63 | 29.38 | 28.62 | 34.73 | 30.37 | 34.16 | 29.11 | 33.53 | 32.61 | 31.87 | 33.29 | – | – | ||
12 | 30.9 | 27.67 | 26.95 | 27.7 | 28.07 | 28.04 | 28.37 | 27.82 | 28.33 | 28.96 | 27.37 | 29.25 | – | – | ||
32–38 | 13 | 32.18 | 28.83 | 29.01 | 29.51 | 29.63 | 30.66 | 29.67 | 28.47 | 30.06 | 30.54 | 28.34 | 30.02 | 33.68 | 36.14 | |
14 | 35.83 | 33.01 | 34.62 | – | – | – | – | – | – | – | – | – | – | – | ||
15 | – | 36.81 | 34.62 | – | – | 34.77 | 32.66 | 36.95 | 34.51 | 36.78 | 32.89 | 35.96 | – | – |
0–3 h | D1 | D2 | D3 | D4 | D5 | D12 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CT | B | R | B | R | B | R | B | R | B | R | B | R | B | R | ||
<25 | 1 | 19.13 | 17.68 | 20.42 | 20.58 | 22.01 | 18.53 | 20.6 | 17.38 | 21.58 | 19.49 | 19.37 | 20.48 | 19.9 | 17.84 | |
2 | 20.09 | 19.68 | 20.12 | 19.81 | 19.52 | 19.16 | 20.11 | 19.15 | 19.38 | 19.66 | 18.78 | 17.76 | 19.51 | 18.38 | ||
3 | 22.99 | 20.82 | 22.64 | 21.95 | 22.09 | 21.25 | 21.47 | 20.67 | 22.81 | 22.63 | 30.26 | 19.5 | 27.29 | 26.88 | ||
4 | 22.05 | 20.92 | 23.71 | 24.57 | 23.54 | 22.48 | 22.67 | 21.81 | 22.28 | 22.56 | 21.56 | 20.44 | 31.05 | 29.74 | ||
5 | 23.89 | 22.75 | 24.03 | 23.5 | 23.4 | 24.6 | 22.63 | 21.81 | 23.44 | 25.35 | 21.66 | 22.61 | – | 34.51 | ||
6 | 24.12 | 21.69 | 23.6 | 24.88 | 23.97 | 23.81 | 26.09 | 23.42 | 24.87 | 24.42 | 23.74 | 22.15 | 27.18 | 25.88 | ||
7 | 24.42 | 22.63 | 24.03 | 23.6 | 23.1 | 22.48 | 23.57 | 20.56 | 23.47 | 24.47 | 21.8 | 22.88 | 29.84 | 30.91 | ||
25–32 | 8 | 25.42 | 23.33 | 25.07 | 24.36 | 23.68 | 23.57 | 23.1 | 24.6 | 24.73 | 23.56 | 21.74 | 23.22 | 25.64 | 24.48 | |
4°C | 9 | 26.36 | 24.61 | 25.05 | 25.39 | 25.39 | 24.42 | 24.26 | 24.35 | 25.01 | 24.42 | 23.49 | 22.77 | 31.78 | – | |
10 | 29.94 | 26.34 | 27.22 | 27.57 | 27.12 | 26.22 | 26.7 | 26.13 | 27.18 | 26.94 | 25.85 | 26.21 | 25.06 | 25.85 | ||
11 | 30.26 | 27.53 | 29.19 | 28.94 | 28.48 | 28.71 | 27.62 | 27.29 | 28.1 | 28.94 | 26.44 | 28.43 | – | – | ||
32–38 | 12 | 34.24 | 26.17 | 28.06 | 30.57 | 28.76 | 27.88 | 33.35 | 29.21 | 31.9 | 38.1 | 29.04 | 30.56 | 29.51 | 29.47 | |
13 | 35.97 | 33.01 | v | – | 34.46 | 33.45 | 33.47 | 33.83 | 33.09 | 32.91 | 32.1 | 33.9 | 31.99 | – | ||
14 | v | 31.83 | 32.9 | 32.45 | 32.28 | 31.36 | 35.1 | 30.34 | 33.4 | 32.28 | 30.01 | 31.92 | – | – | ||
15 | – | – | 34.82 | 34.26 | ‐ | 34.68 | –‐ | 34.96 | – | – | 33.8 | – | – | – |
Abbreviations: B, Biorad CFX96; R, Roche Light Cycler 480.