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Schematic representation of selected assays for GTase activity. A) Radioactive assay based on two steps of guanylyltransferase reaction: 1) formation of GpE covalent complex and 2) GMP transfer to 5′ RNA diphosphate. Illustration was prepared using Inkscape software based on the experimental results of Soulière et al. 2008. B) ELISA assay for GTase characterization. C) Fluorescence polarization assay based on BODIPY‐labelled GTP.
