Table 2.
Target of PCR | Primer/Probe | Sequences (5ʹ–3ʹ) |
---|---|---|
A23403G | Upstream primer | TGTTCTTTTGGTGGTGTCAGT |
Downstream primer | TAGAATAAACACGCCAAGTAGGAGT | |
6‐FAM‐probe | TTCTTTATCAGGATGTTAACTGCACAGAA‐6FAM | |
LCRed 640 probe | LCR640‐TCCCTGTTGCTATTCATGCAGATCA‐PHOSPATE | |
C14408T | Upstream primer | TTAAATATTGGGATCAGACATACC |
Downstream primer | GAAGTGGTATCCAGTTGAAACT | |
6‐FAM‐probe | AAAACTTGTAAGTGGGAACACTGT ‐6FAM | |
LCRed 640 probe | LCR640‐ GAGAATAAAACATTAAAGTTTGCA‐phosphate |
Abbreviations: ATCC, american type culture collection; 6‐FAM, 6‐carboxyfluorescein; PCR, polymerase chain reaction.