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. 2021 Jul 10;8:309–324. doi: 10.1016/j.bioactmat.2021.07.002

Fig. 5.

Fig. 5

In vitro osteogenic differentiation. (A) ALP staining of BM-MSCs on different samples after 14 days of osteogenic induction (Scale bar = 100 μm). (B) ARS staining of BM-MSCs on different samples after 21 days of osteogenic induction (Scale bar = 100 μm). (C) Quantitative analysis of ALP activity in different groups after 4, 7 and 14 days of osteogenic induction (n = 6 per group). (D) Semi-quantitative analysis of ARS staining in different groups after 7, 14 and 21 days of osteogenic induction (n = 6 per group). (E–H) Gene expressions of osteogenesis-related proteins including ALP, Runx2, COL1A1 and OCN of BM-MSCs in different groups after 4, 7 and 14 days of osteogenic induction (n = 6 per group). Statistically significant differences were indicated by *p < 0.05 and #p < 0.01 compared with the bare PEEK group.