Fig. 8.

In vivo osteogenic assessments after 6 weeks of implantation. (A–C) H&E staining, Toluidine blue staining and Calcein double-labeling of the peri-implant tissues in different groups (scale bar = 100 μm). (D, E) Immunofluorescent staining of the peri-implant tissues in different groups in red (Osterix, OCN) and blue (nuclei) fluorescence with white dotted lines indicating the contour distribution (scale bar = 50 μm). (F–J) Quantitative analysis of the thickness of fibrous capsule, BIC, MAR and percentage of Osterix and OCN positive cells (n = 6 per group). Statistically significant differences were indicated by *p < 0.05 and ^#p < 0.01 compared with the bare PEEK group.