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. 2021 Aug 26;11:679262. doi: 10.3389/fonc.2021.679262

Figure 6.

Figure 6

In vitro experiments confirmed that OXPHOS plays an important role in breast cancer metastases. (A) Schematic diagram of experimental plan for determining the effect of oligomycin treatment on MDA-MB-231 cells. Oligo(1.0) = 1 μM oligomycin. (B, C) The effect of Oligo(1.0) on the proliferation and growth rate of MDA-MB-231 cells measured by CCK8. The calculation method of growth rate was as follows: Growth rate of Control = ABS(OD value of Control – mean(OD value of Control group)) / mean(OD value of Control group), Growth rate of Oligo(1.0) = ABS(OD value of Oligo(1.0) - mean(OD value of Control group)) / mean(OD value of Control group). (D) Representative images of flow cytometry analysis of MDA-MB-231 cells treated with Oligo(1.0) after 48h. (E) Representative images of 48h wound healing rate of scratched wounds of MDA-MB-231 cells treated with Oligo(1.0). Scale bars = 200um. (F) Representative images of 24h migrating and invasion results of MDA-MB-231 cells treated with Oligo(1.0). Scale bars = 50 um. *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant, P values determined by unpaired two-sided Student’s t-test. The data is expressed as the mean ± sd for n =3 replicates.