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E4F262 does not inhibit E1A expression from pβA-E1A(13S). Shown are Western blots of lysates from NIH 3T3 cells transfected with E1A expression plasmid pβA-E1A(13S), p50E4F expression plasmid pCMVs-E4F262, or pβA-E1A(13S) plus pCMVs-E4F262; a duplicate set of transfections contained the activated ras expression plasmid pSP72-ras (+ Ras). Polyclonal antibodies against the E1A(13S) product were used to detected E1A protein [E1A (289R)].
