FIG. 3.

E4F262 truncation mutants. (A) Schematic depiction of E4F262, N-terminal deletion mutants (N1, N2, and N5), and C-terminal deletion mutants (C1, C2, and C3) used in this study; E4F262 residues that remain in each truncation mutant are indicated by black bars and denoted in parentheses. (B) Western blot of lysates from NIH 3T3 cells transfected with pCMVs constructs expressing wild-type (WT) E4F262 and the truncation mutants. E4F-N4-specific antiserum was used to detect E4F proteins (marked by arrowheads); E4F-N5 and E4F-C3 (not shown) proteins do not contain the epitope recognized by this antiserum.