Fig. 5.

Efficacy on anti-inflammatory properties of Gastrodin/PU scaffolds. (A–C) Scaffolds incubated with Raw264.7 cells for 3 days: (A) Staining cytoplasm (iNOS, red) and nucleus (blue) of cells; (B) RT-qPCR analysis; (C) The content of TNF-ɑ protein. (D–F) Host response to the scaffolds following 14-day subcutaneous implantation: (D) Immunofluorescent (TNF-α) staining; (E) Masson staining; (F) Quantitative results of thickness of fibrous capsules from Masson staining. “F” denotes fibrous capsules; two-way green arrows mark the span of the fibrous capsule at the film-tissue interface. Data are expressed as the mean ± SD (n = 5). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)