Fig. 1.

In vitro QC steps used to manufacture NS/PCs from YZWJs513 iPSCs. (a) Images of iPSCs, embryoid bodies (EBs, intermediate product), and NS/PCs (scale bar = 200 μm). (b) During culture, the passage number, days of culture, and number of cell divisions were monitored and all within the threshold. (c) ICC of the final product showed no expression of OCT4 (marker of pluripotency). More than 90% of cells expressed SOX1 and Nestin (markers of NS/PCs; scale bar = 100 μm). (d) Chromosomal analysis of the final product (P4) and the longer culture product (P8) yielded normal results. (e) ICC after 4 weeks of differentiation culture showed the potential of NS/PCs to differentiate into β–III–tubulin-positive neurons and GFAP-positive astrocytes. Proliferation of OCT4-positive cells was not observed after culture under pluripotent stem cell culture conditions (scale bar = 100 μm).