Fig. 3.

Representative histological images of axial brain section of NOG mouse at 6 months after the transplantation of 1 × 10⁶ cells of YZWJs513 hiPSC-NS/PCs for clinical use to each striatum. (a) Arrows indicate the transplantation sites, and the cell engraftment site is observed as light-colored area by H-E staining. (b) Enlarged image from a (c–g) Immunostained images of the same area as b: human nuclear antigen (c); Ki67 (d); STEM121 (human cytoplasm antigen) (e); human specific Nestin (f); and STEM123 (human specific GFAP) (g). The cells in the transplanted cites were uniform regardless of the engraftment site such as the brain surface, the striatum, and the white matter, and almost no anti-Nestin antibody staining-positive cells were observed, and the differentiation tendency was STEM123 staining-positive. In addition, the anti-Ki67 antibody staining positive cell rate was lower than 5%, and it was considered that the proliferative ability and neoplasticity were negligible.