Using the qPCR method to test VDR in a Salmonella-colitis model. (A) VDR staining in colon of Salmonella-treatment mice. Images were representative of experiments in triplicate; Control, n = 6; Salmonella, n = 8. (B) Comparison of RT-PCR expression profiles of VDR gene from paired Salmonella-treated and control fecal samples. VDR expression was down-regulated in feces from chronic Salmonella infected mice (4 days post infection). Data are expressed as mean ± SD. n = 6, Student's t-test, ∗P < 0.05. (C) Comparison of qPCR expression profiles of Vdr gene from paired Salmonella-treated and control intestinal samples. VDR expression was down-regulated in Salmonella-treated mice intestinal samples. Data are expressed as mean ± SD. n = 6, Student's t-test, ∗P < 0.05.