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. 2020 Dec 23;8(6):891–906. doi: 10.1016/j.gendis.2020.12.006

Figure 2.

Fig. 2

Mitochondrial respiratory function increased after the differentiation of hiPSCs into cardiomyocytes. (A) Representative two OCR traces of day 0-hiPSCs and day 30-hiPSC-CMs respectively, in response to oligomycin, FCCP, and antimycin A. (B) OCR parameters representing mitochondrial function in day 0-hiPSCs and day 30-hiPSC-CMs. (C) The cellular ATP content of day 0-hiPSCs and day 30-hiPSC-CMs was measured using a bioluminescent assay system. (D) Mitochondrial membrane potential analysis was measured using a fluorescence probe JC-1 assay system in day 0-hiPSCs and day 30-hiPSC-CMs. The ratio of red/green fluorescence represented the level of Δψm. (E) The expression of PGC-1α and its downstream metabolic-related proteins increased after hiPSCs differentiate into cardiomyocytes. Protein bands were detected by Western blot analysis, and the proteins assessed were PGC-1α, NRF1, ERRα, CPT1α, CPT1β, CS, COXⅣ, COX5B, CytC and MPC1. Compared with hiPSCs group ∗P < 0.05.